crispr cas12a trans cleavage assay (New England Biolabs)
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crispr cas12a trans cleavage assay
Crispr Cas12a Trans Cleavage Assay, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas12a/NEBuffer+2/pmc12962081-97-1-17
Average 99 stars, based on 2870 article reviews
Crispr Cas12a Trans Cleavage Assay, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas12a/NEBuffer+2/pmc12962081-97-1-17
Average 99 stars, based on 2870 article reviews
crispr cas12a trans cleavage assay - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "Field-deployable multiplex RAA-CRISPR/Cas12a platform rapidly and simultaneously detects seven Eimeria species in chickens"
Article Title: Field-deployable multiplex RAA-CRISPR/Cas12a platform rapidly and simultaneously detects seven Eimeria species in chickens
Journal: Poultry Science
doi: 10.1016/j.psj.2026.106681
Figure Legend Snippet: Screening of species-specific crRNAs and determination of fluorescence and visual detection thresholds for the E-MRC12a assay. (A-G) Real-time fluorescence curves of CRISPR/Cas12a reactions targeting the 18S rDNA of the seven Eimeria species using three candidate crRNAs per species: (A) E. maxima (EM1-EM3), (B) E. acervulina (EA1-EA3), (C) E. necatrix (EN1-EN3), (D) E. mitis (EI1-EI3), (E) E. praecox (EP1-EP3), (F) E.brunelti (EB1-EB3) and (G) E. tenella (ET1). (H) UV images of CRISPR/Cas12a reaction products for each species-specific crRNA. (I) Determination of the visual fluorescence threshold. NT: no-template control. Error bars represent mean ± SD (n = 3 per group).
Techniques Used: Fluorescence, CRISPR, Control
Figure Legend Snippet: Evaluation of the specificity, sensitivity, and repeatability of the E-MRC12a assay . (A) Heatmap of fluorescence intensities from CRISPR/Cas12a reactions using species-specific crRNAs targeting genomic DNA from the seven Eimeria species, Escherichia coli, Salmonella spp ., and confirmed Eimeria -negative fecal samples. (B) Visual fluorescence under 254 nm UV light corresponding to (A). (C) Heatmap showing the sensitivity evaluation of the E-MRC12a assay. (D) Visual fluorescence under 254 nm UV light corresponding to (C). (E) Heatmap illustrating repeatability assessment of the E-MRC12a assay. NT: No-template control. For panels (A) and (C), different letters within a row indicate statistically significant differences ( P < 0.05); identical letters indicate no significant difference ( P > 0.05).
Techniques Used: Fluorescence, CRISPR, Control
Figure Legend Snippet: Schematic view of the nucleic acid extraction and Eimeria –Multiplex RAA–CRISPR/Cas12a (E-MRC12a) typing assay.
Techniques Used: Extraction, Multiplex Assay, CRISPR
Related Articles
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Article Title: CRISPR/Cas12a and fork-shaped probe enhance LAMP–LFT integration for equipment-free detection of Listeria monocytogenes Article Snippet: Listeria monocytogenes is an important foodborne pathogen associated with high mortality rates, especially among vulnerable populations, and therefore requires diagnostic methods that are not only highly sensitive and rapid but also suitable for use in resource-limited settings.. In this study, we developed an isothermal amplification assay integrated with a lateral flow test (LFT) for reliable detection of L. monocytogenes.. Two assay formats were designed and compared: (1) loop-mediated isothermal amplification (LAMP) with LFT detection of fluoresceinand biotin-labeled amplicons, and (2) LAMP combined with CRISPR/Cas12a, using LFT to detect a cleaved forkshaped enhanced probe labeled with three fluoresceins. Article Title: Breaking the PAM Restriction: A Universal Double Stranded DNA Detection Method Based on the Sticky End-Mediated CRISPR/Cas12a Coupled RPA and Its Application to KRAS G12C Single Base Mutations. Article Snippet: The CRISPR/Cas12a system facilitates efficient and specific nucleic acid detection, but its dependence on Protospacer Adjacent Motif (PAM) sequences and the complexity of existing sticky end-based methods pose challenges for stable and portable applications.. To address these issues, this study developed a universal dsDNA detection method by integrating the sticky endmediated CRISPR/Cas12a with recombinase polymerase amplification (RPA).. By incorporating NlaIII recognition sites into RPA primers, precise cleavage of amplification products was achieved, generating uniform sticky ends and eliminating reliance on PAM sites. Incubation:Article Title: Self-priming amplification-integrated orthogonal CRISPR-Cas system for multiplexed profiling of piRNAs in clinical samples. Article Snippet: CRISPR/Cas-based biosensors hold great diagnostic potential, but they are often limited in clinical practice by insufficient sensitivity and the lack of multiplexed analysis capability.. To address these issues, we develop a selfpriming amplification-integrated orthogonal CRISPR-Cas (SPA-OCRISPR) system for multiplexed detection of piRNAs.. This assay employs an innovative four-way junction probe that seamlessly integrates target recognition with a primer-free self-priming amplification, significantly simplifying the workflow and enhancing the ligation efficiency. Article Title: CRISPR/Cas12a and fork-shaped probe enhance LAMP–LFT integration for equipment-free detection of Listeria monocytogenes Article Snippet: Listeria monocytogenes is an important foodborne pathogen associated with high mortality rates, especially among vulnerable populations, and therefore requires diagnostic methods that are not only highly sensitive and rapid but also suitable for use in resource-limited settings.. In this study, we developed an isothermal amplification assay integrated with a lateral flow test (LFT) for reliable detection of L. monocytogenes.. Two assay formats were designed and compared: (1) loop-mediated isothermal amplification (LAMP) with LFT detection of fluoresceinand biotin-labeled amplicons, and (2) LAMP combined with CRISPR/Cas12a, using LFT to detect a cleaved forkshaped enhanced probe labeled with three fluoresceins. CRISPR:Article Title: Self-priming amplification-integrated orthogonal CRISPR-Cas system for multiplexed profiling of piRNAs in clinical samples. Article Snippet: CRISPR/Cas-based biosensors hold great diagnostic potential, but they are often limited in clinical practice by insufficient sensitivity and the lack of multiplexed analysis capability.. To address these issues, we develop a selfpriming amplification-integrated orthogonal CRISPR-Cas (SPA-OCRISPR) system for multiplexed detection of piRNAs.. This assay employs an innovative four-way junction probe that seamlessly integrates target recognition with a primer-free self-priming amplification, significantly simplifying the workflow and enhancing the ligation efficiency. 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<i>Mycobacterium tuberculosis</i> Article Snippet: .. Next, 1.0 μL of Polymerase Chain Reaction:Article Title: CRISPR/Cas12a and fork-shaped probe enhance LAMP–LFT integration for equipment-free detection of Listeria monocytogenes Article Snippet: Listeria monocytogenes is an important foodborne pathogen associated with high mortality rates, especially among vulnerable populations, and therefore requires diagnostic methods that are not only highly sensitive and rapid but also suitable for use in resource-limited settings.. In this study, we developed an isothermal amplification assay integrated with a lateral flow test (LFT) for reliable detection of L. monocytogenes.. Two assay formats were designed and compared: (1) loop-mediated isothermal amplification (LAMP) with LFT detection of fluoresceinand biotin-labeled amplicons, and (2) LAMP combined with CRISPR/Cas12a, using LFT to detect a cleaved forkshaped enhanced probe labeled with three fluoresceins. SYBR Green Assay:Article Title: CRISPR/Cas12a and fork-shaped probe enhance LAMP–LFT integration for equipment-free detection of Listeria monocytogenes Article Snippet: Listeria monocytogenes is an important foodborne pathogen associated with high mortality rates, especially among vulnerable populations, and therefore requires diagnostic methods that are not only highly sensitive and rapid but also suitable for use in resource-limited settings.. In this study, we developed an isothermal amplification assay integrated with a lateral flow test (LFT) for reliable detection of L. monocytogenes.. Two assay formats were designed and compared: (1) loop-mediated isothermal amplification (LAMP) with LFT detection of fluoresceinand biotin-labeled amplicons, and (2) LAMP combined with CRISPR/Cas12a, using LFT to detect a cleaved forkshaped enhanced probe labeled with three fluoresceins. Recombinase Polymerase Amplification:Article Title: Breaking the PAM Restriction: A Universal Double Stranded DNA Detection Method Based on the Sticky End-Mediated CRISPR/Cas12a Coupled RPA and Its Application to KRAS G12C Single Base Mutations. Article Snippet: The CRISPR/Cas12a system facilitates efficient and specific nucleic acid detection, but its dependence on Protospacer Adjacent Motif (PAM) sequences and the complexity of existing sticky end-based methods pose challenges for stable and portable applications.. To address these issues, this study developed a universal dsDNA detection method by integrating the sticky endmediated CRISPR/Cas12a with recombinase polymerase amplification (RPA).. By incorporating NlaIII recognition sites into RPA primers, precise cleavage of amplification products was achieved, generating uniform sticky ends and eliminating reliance on PAM sites. Article Title: Development of a Sensitive and Specific RPA-CRISPR/Cas12a Assay for Intrahepatic Quantification of HBV cccDNA Article Snippet: CRISPR/Cas12a-based detection was conducted using EnGen ® Lba Cas12a (NEB, Ipswich, MA, USA), synthetic crRNAs and ssDNA fluorescent reporters (BIONICS, Seoul, Republic of Korea). .. Final 100 μL reactions contained: 1× NEBuffer r2.1, 30 nM Irradiation:Article Title: Mismatch-Driven CRISPR/Cas12a Biosensing of UV-Induced DNA Lesions for Environmental Solar Exposure Surveillance. Article Snippet: .. Following irradiation, the DNA solution was kept under ambient temperature and light conditions for approximately 10 min and then combined at a 1:1 volume ratio with a master mix containing NEBuffer r2.1, crRNA, Plasmid Preparation:Article Title: Development of a Sensitive and Specific RPA-CRISPR/Cas12a Assay for Intrahepatic Quantification of HBV cccDNA Article Snippet: CRISPR/Cas12a-based detection was conducted using EnGen ® Lba Cas12a (NEB, Ipswich, MA, USA), synthetic crRNAs and ssDNA fluorescent reporters (BIONICS, Seoul, Republic of Korea). .. Final 100 μL reactions contained: 1× NEBuffer r2.1, 30 nM Activity Assay:Article Title: CRISPR in MOF Formulation with Enhanced Stability, Activity, and Altered PAM Specificity for Broad-Spectrum Diagnosis of Bacterial Sepsis. Article Snippet: .. While Mg is typically the preferred cofactor for |